This book presents basic concepts, methodologies and applications of biotechnology for the conservation and propagation of aromatic, medicinal and other economic plants. It caters to the needs and challenges of researchers in plant biology, biotechnology, the medical sciences, pharmaceutical biotechnology and pharmacology areas by providing an accessible and cost-effective practical approach to micro-propagation and conservation strategies for plant species. It also includes illustrations describing a complete documentation of the results and research into particular plant species conducted by the authors over the past 5 years. Plant Biotechnology has been a subject of academic interest for a considerable time. In recent years, it has also become a useful tool in agriculture and medicine, as well as a popular area of biological research. Current economic growth is globally projected in a highly positive manner, but the challenges many countries face with regard to food, feed, malnutrition, infectious diseases, the newly identified life-style diseases, and energy shortages, all of which are worsened by an ever-deteriorating environment, continue to pull the growth digits back. The common thread that connects all of the above challenges is biotechnology, which could provide many answers. Molecular biology and biotechnology have now become an integral part of tissue culture research. The tremendous impact generated by genetic engineering and consequently of transgenics now allows us to manipulate plant genomes at will. There has indeed been a rapid development in this area with major successes in both developed and developing countries. The book introduces several new and exciting areas to researchers who are unfamiliar with plant biotechnology and also serves as a review of ongoing research and future directions for scholars. The book highlights numerous methods for in vitro propagation and utilization of techniques in raising transgenics to help readers reproduce the experiments discussed.

Plant tissue culture is an essential component of Biotechnology which has gained unbeatable recognition in plant sciences for successful micropropagation and improvement of plant species, leading to the commercial application. A number of plant species have been investigated around the globe. This book presents current research on the application of in vitro technology in the improvement of Balanites aegyptiaca Del., a medicinal plant of semi-arid tropics. The worldwide importance of forestry, summed to the lengthy generation cycles of tree species, makes unavoidable development of new technologies that complement conventional tree breeding programmes in order to obtain improved genotypes. Recently, a new set of tools has become available in the past 20 years that combined with traditional plant breeding will allow scientists to generate products that are genetically improved varieties of the future. These set of tools come under the general title of ‘Biotechnology’. The three specific biotechnological tools have been successfully used in several programmes of plant conservation, namely, tissue culture techniques for in vitro propagation, the use of molecular markers to assess the degree of variability among population and techniques of long-term conservation such as encapsulation and cryopreservation. Plant tissue culture techniques are particularly relevant and become an alternative not only for large scale propagation of individuals that are threatened, reduce production costs and increase gains to the industry, but also to provide ecological advantages as in phytoremediation or in the establishment of artificial plantings in weed infested site. The book gives a complete documentation of the results and demonstration of Balanites aegyptiaca conducted by the authors over the past 5 years. The end-to-end approach developed through plant tissue culture techniques is reflected in the book and there has been a successful transfer of technology from lab to field. The authors hope that this information would provide valuable data and also be a reference material for future research activities in this area.

This book presents basic concepts, methodologies and applications of biotechnology for the conservation and propagation of aromatic, medicinal and other economic plants. It caters to the needs and challenges of researchers in plant biology, biotechnology, the medical sciences, pharmaceutical biotechnology and pharmacology areas by providing an accessible and cost-effective practical approach to micro-propagation and conservation strategies for plant species. It also includes illustrations describing a complete documentation of the results and research into particular plant species conducted by the authors over the past 5 years. Plant Biotechnology has been a subject of academic interest for a considerable time. In recent years, it has also become a useful tool in agriculture and medicine, as well as a popular area of biological research. Current economic growth is globally projected in a highly positive manner, but the challenges many countries face with regard to food, feed, malnutrition, infectious diseases, the newly identified life-style diseases, and energy shortages, all of which are worsened by an ever-deteriorating environment, continue to pull the growth digits back. The common thread that connects all of the above challenges is biotechnology, which could provide many answers. Molecular biology and biotechnology have now become an integral part of tissue culture research. The tremendous impact generated by genetic engineering and consequently of transgenics now allows us to manipulate plant genomes at will. There has indeed been a rapid development in this area with major successes in both developed and developing countries. The book introduces several new and exciting areas to researchers who are unfamiliar with plant biotechnology and also serves as a review of ongoing research and future directions for scholars. The book highlights numerous methods for in vitro propagation and utilization of techniques in raising transgenics to help readers reproduce the experiments discussed.

Medicinal plants have acquired increasing significance in development co-operation over the last few years. Their use and propagation are cross-sectoral concerns that embrace not only health-care but also nature conservation, biodiversity, economic assistance, trade and legal aspects (e.g. intellectual property). The present book presents biotechnological approaches for the in vitro conservation and mass propagation of two important medicinal plants viz; Tylophora indica and Rauvolfia tetraphylla. Both the plants are rapidly disappearing due to its extensive indiscriminate collection from wild, poor seed germination and lack of adequate commercial plantation. Regeneration and establishment of plantlets through various plant tissue culture techniques has been discussed in this book. The application of these protocols can help to minimize the pressure on wild populations and contribute to the conservation of these valuable medicinal plants. Regeneration of plants from alginate encapsulated shoot buds could be used as nodal delivery system for germplasm storage and exchange. Physiological and biochemical changes during ex vitro establishment micropropagated plants has been discussed.

Plant tissue culture is an essential component of Biotechnology which has gained unbeatable recognition in plant sciences for successful micropropagation and improvement of plant species, leading to the commercial application. A number of plant species have been investigated around the globe. This book presents current research on the application of in vitro technology in the improvement of Balanites aegyptiaca Del., a medicinal plant of semi-arid tropics. The worldwide importance of forestry, summed to the lengthy generation cycles of tree species, makes unavoidable development of new technologies that complement conventional tree breeding programmes in order to obtain improved genotypes. Recently, a new set of tools has become available in the past 20 years that combined with traditional plant breeding will allow scientists to generate products that are genetically improved varieties of the future. These set of tools come under the general title of Biotechnology . The three specific biotechnological tools have been successfully used in several programmes of plant conservation, namely, tissue culture techniques for in vitro propagation, the use of molecular markers to assess the degree of variability among population and techniques of long-term conservation such as encapsulation and cryopreservation. Plant tissue culture techniques are particularly relevant and become an alternative not only for large scale propagation of individuals that are threatened, reduce production costs and increase gains to the industry, but also to provide ecological advantages as in phytoremediation or in the establishment of artificial plantings in weed infested site. The book gives a complete documentation of the results and demonstration of Balanites aegyptiaca conducted by the authors over the past 5 years. The end-to-end approach developed through plant tissue culture techniques is reflected in the book and there has been a successful transfer of technology from lab to field. The authors hope that this information would provide valuable data and also be a reference material for future research activities in this area."